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green fluorescent protein fusion  (OriGene)


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    Structured Review

    OriGene green fluorescent protein fusion
    Green Fluorescent Protein Fusion, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+protein+gfp/pLenti-EF1a-C-mGFP+Lentiviral+Gene+Expression+Vector/pm42271190-47-26-29
    Average 94 stars, based on 6 article reviews
    green fluorescent protein fusion - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Control:

    Article Title: Ex vivo reprogramming of human hematopoietic stem cells is accompanied by increased transcripts of genes regulating metabolic integrity
    Article Snippet: .. Plasmids and Production of Lentiviral Particles Short-hairpin (sh) RNA lentiviral plasmids targeting SIRT1 (SIRT1shRNA, TL309433), SIRT3 (SIRT3-shRNA, TL309432), or scrambled (control-shRNA, TR30021) that are also encoded with a green fluorescent protein (GFP) were purchased from Origene. ..

    Labeling:

    Article Title: Klotho enhances stability of chronic kidney disease atherosclerotic plaques by inhibiting GRK2/PLC-β-mediated endoplasmic reticulum stress in macrophages via modulation of the ROS/SHP1 pathway
    Article Snippet: .. Lentiviral vectors carrying full-length mouse Klotho cDNA (LV-KL) labeled with green fluorescent protein (GFP) were obtained from OriGene Technologies and accompanied by a negative control. .. The lentiviral particles were then packaged by GeneChem.

    Negative Control:

    Article Title: Klotho enhances stability of chronic kidney disease atherosclerotic plaques by inhibiting GRK2/PLC-β-mediated endoplasmic reticulum stress in macrophages via modulation of the ROS/SHP1 pathway
    Article Snippet: .. Lentiviral vectors carrying full-length mouse Klotho cDNA (LV-KL) labeled with green fluorescent protein (GFP) were obtained from OriGene Technologies and accompanied by a negative control. .. The lentiviral particles were then packaged by GeneChem.

    Expressing:

    Article Title: Identification of novel CD44v6-binding peptides that block CD44v6 and deliver a pro-apoptotic peptide to tumors to inhibit tumor growth and metastasis in mice
    Article Snippet: .. The green fluorescent protein (GFP)-tagged mammalian expression vector pCMV6-AC-GFP containing CD44v6 open reading frame was purchased from Origene (catalogue no. RG234071, Rockville, MD). ..

    Plasmid Preparation:

    Article Title: Identification of novel CD44v6-binding peptides that block CD44v6 and deliver a pro-apoptotic peptide to tumors to inhibit tumor growth and metastasis in mice
    Article Snippet: .. The green fluorescent protein (GFP)-tagged mammalian expression vector pCMV6-AC-GFP containing CD44v6 open reading frame was purchased from Origene (catalogue no. RG234071, Rockville, MD). ..

    Article Title: Sweet-bitter taste interactions in binary mixtures of sweeteners: Relationship between taste receptor activities and sensory perception.
    Article Snippet: They were maintained in an incubator (Thermo ScientificTM BB15 CO2 Incubator, Waltham, MA, USA) at 37 ◦C with 5% CO2. .. To establish an in vitro human taste receptor affinity test system, recombinant plasmid constructs, specifically pCMV6-Entry containing TAS1R3 and pCMV6-Entry containing TAS1R2 fused with green fluorescent protein (GFP) (OriGene, Rockville, MD, USA), were constructed. ..

    Article Title: MRGPRX2-Mediated Mast Cell Degranulation by Monomethyl Methacrylate: Unveiling a Pathway in Bone Cement Implantation Syndrome.
    Article Snippet: Bone cement implantation syndrome is a critical complication of orthopaedic surgery, characterised by hypotension and hypoxemia.. This syndrome is hypothesised to result from obstruction caused by fat droplets and the biochemical release of histamine caused by bone cement components.. This study aimed to elucidate the histamine release mechanism, focusing on Masrelated G proteincoupled receptor X2 expressed on mast cells, which is hypothesised to be activated by bone cement components.

    Transduction:

    Article Title: Endothelial Overexpression of TGF-β-Induced Protein Impairs Venous Thrombus Resolution
    Article Snippet: Forty-eight hours later, cells were either fixed in 4% paraformaldehyde for immunofluorescence staining with primary antibody (polyclonal TGFBI antibody ab99562; Abcam) or lysed in Trizol for mRNA isolation. .. HPAECs were transduced with human TGFBI ORF tagged with green fluorescent protein (GFP) (OriGene RC200411L2V) or stimulated with recombinant TGFBI (50 ng/μL; Biozol USB-157133) mixed with human venous whole blood and allowed to clot. .. Human aortic adventitial fibroblasts (HAoAFs) (PromoCell) were used as control.

    Recombinant:

    Article Title: Endothelial Overexpression of TGF-β-Induced Protein Impairs Venous Thrombus Resolution
    Article Snippet: Forty-eight hours later, cells were either fixed in 4% paraformaldehyde for immunofluorescence staining with primary antibody (polyclonal TGFBI antibody ab99562; Abcam) or lysed in Trizol for mRNA isolation. .. HPAECs were transduced with human TGFBI ORF tagged with green fluorescent protein (GFP) (OriGene RC200411L2V) or stimulated with recombinant TGFBI (50 ng/μL; Biozol USB-157133) mixed with human venous whole blood and allowed to clot. .. Human aortic adventitial fibroblasts (HAoAFs) (PromoCell) were used as control.

    Article Title: Sweet-bitter taste interactions in binary mixtures of sweeteners: Relationship between taste receptor activities and sensory perception.
    Article Snippet: They were maintained in an incubator (Thermo ScientificTM BB15 CO2 Incubator, Waltham, MA, USA) at 37 ◦C with 5% CO2. .. To establish an in vitro human taste receptor affinity test system, recombinant plasmid constructs, specifically pCMV6-Entry containing TAS1R3 and pCMV6-Entry containing TAS1R2 fused with green fluorescent protein (GFP) (OriGene, Rockville, MD, USA), were constructed. ..

    In Vitro:

    Article Title: Sweet-bitter taste interactions in binary mixtures of sweeteners: Relationship between taste receptor activities and sensory perception.
    Article Snippet: They were maintained in an incubator (Thermo ScientificTM BB15 CO2 Incubator, Waltham, MA, USA) at 37 ◦C with 5% CO2. .. To establish an in vitro human taste receptor affinity test system, recombinant plasmid constructs, specifically pCMV6-Entry containing TAS1R3 and pCMV6-Entry containing TAS1R2 fused with green fluorescent protein (GFP) (OriGene, Rockville, MD, USA), were constructed. ..

    Construct:

    Article Title: Sweet-bitter taste interactions in binary mixtures of sweeteners: Relationship between taste receptor activities and sensory perception.
    Article Snippet: They were maintained in an incubator (Thermo ScientificTM BB15 CO2 Incubator, Waltham, MA, USA) at 37 ◦C with 5% CO2. .. To establish an in vitro human taste receptor affinity test system, recombinant plasmid constructs, specifically pCMV6-Entry containing TAS1R3 and pCMV6-Entry containing TAS1R2 fused with green fluorescent protein (GFP) (OriGene, Rockville, MD, USA), were constructed. ..

    Transfection:

    Article Title: MRGPRX2-Mediated Mast Cell Degranulation by Monomethyl Methacrylate: Unveiling a Pathway in Bone Cement Implantation Syndrome.
    Article Snippet: Bone cement implantation syndrome is a critical complication of orthopaedic surgery, characterised by hypotension and hypoxemia.. This syndrome is hypothesised to result from obstruction caused by fat droplets and the biochemical release of histamine caused by bone cement components.. This study aimed to elucidate the histamine release mechanism, focusing on Masrelated G proteincoupled receptor X2 expressed on mast cells, which is hypothesised to be activated by bone cement components.



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    OriGene fluorescent protein gfp reporter
    (A) Representative <t>fluorescent</t> images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of <t>GFP-expressing</t> RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.
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    OriGene green fluorescent protein gfp
    (A) Representative <t>fluorescent</t> images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of <t>GFP-expressing</t> RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.
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    Image Search Results


    (A) Representative fluorescent images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of GFP-expressing RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.

    Journal: Journal of Nuclear Medicine

    Article Title: PD-L1 Immuno-PET Reveals Systemic Effects of Localized Oncolytic Virotherapy in a Mouse Model of Head and Neck Cancer

    doi: 10.2967/jnumed.125.270922

    Figure Lengend Snippet: (A) Representative fluorescent images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of GFP-expressing RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.

    Article Snippet: The MOC2( PD-L1 ) cell line was generated in our laboratory to overexpress PD-L1 by transducing MOC2 cells with a lentiviral vector carrying the murine CD274 gene tagged with a green fluorescent protein (GFP) reporter (MR 203953L2; OriGene Technologies).

    Techniques: Incubation, Expressing, In Vitro, Glo Assay